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ST Holding Medium (m-ST Holding Medium)

[所屬分類:培養基配方] [發布時間:2021-12-17] [發布人:網站管理員2] [閱讀次數:] [返回]
ST Holding Medium
(m-ST Holding Medium)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

KH2PO4...............................................................3.0g
Tris(hydroxymethyl)aminomethane buffer.........3.0g
Sulfanilamide......................................................1.5g
NaH2PO4·H2O.....................................................0.1g
Ethanol (95% solution) ................................. 10.0mL
pH 8.6 ± 0.2 at 25°C
Preparation of Medium: Dissolve the sulfanil
amide in the ethanol. Add all components to distilled/
deionized water and bring volume to 1.0L. Mix thor
oughly. Autoclave for 15 min at 15 psi pressure–
121°C. Distribute in 1.8mL volumes to sterile Petri
dishes with tight-fitting lids and an absorbent filter.
Use: For the cultivation and enumeration of coliform
bacteria by the delayed-incubation total coliform
procedure. For use as a holding or transport medium
to keep coliform bacteria viable between sampling
and laboratory culture.
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