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STAA Agar Base

[所屬分類:培養基配方] [發布時間:2021-12-17] [發布人:網站管理員2] [閱讀次數:] [返回]
STAA Agar Base

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Peptone .............................................................20.0g
Agar ..................................................................13.0g
Glycerol ..............................................................7.5g
Yeast extract........................................................2.0g
K2HPO4...............................................................1.0g
MgSO4·7H2O......................................................1.0g
STAA selective supplement.......................... 10.0mL
pH 7.0 ± 0.1 at 25°C
Source: This medium is available as a premixed
powder from Oxoid.
STAA Selective Supplement:
Composition per 10.0mL:
Streptomycin sulfate ...........................................0.5g
Cycloheximide..................................................0.05g
Thallous acetate ................................................0.05g
Preparation of STAA Selective Supplement:
Add components to distilled/deionized water and
bring volume to 10.0mL. Mix thoroughly. Filter ster
ilize.
Preparation of Medium: Add components, ex
cept glycerol and STAA selective supplement, to dis
tilled/deionized water and bring volume to 985.0mL.
Mix thoroughly. Gently heat and bring to boiling.
Add glycerol. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti
cally add sterile STAA selective supplement. Mix
thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the isolation of Brochothrix thermosphacta
from meat products.
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