培養基配方

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SSM Agar

[所屬分類:培養基配方] [發布時間:2021-12-17] [發布人:網站管理員2] [閱讀次數:] [返回]

SSM Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Solution A................................................... 850.0mL

Solution C ................................................... 100.0mL

Solution B ..................................................... 50.0mL

Solution A:

Composition per 850.0mL:

Agar, noble........................................................15.0g

Na2HPO4 .............................................................6.0g

KH2PO4...............................................................3.0g

MgSO4·7H2O ......................................................0.2g

Preparation of Solution A: Add components to

distilled/deionized water and bring volume to

850.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°–55°C.

Solution B:

Composition per 50.0mL:

Glucose ...............................................................5.0g

Preparation of Solution B: Add glucose to dis

tilled/deionized water and bring volume to 50.0mL.

Mix thoroughly. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 50°–55°C.

Solution C:

Composition per 100.0mL:

Casein hydrolysate............................................20.0g

Preparation of Solution C: Add casein hydroly

sate to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter through Whatman

#1 paper. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°–55°C.

Preparation of Medium: Aseptically combine

850.0mL of sterile solution A, 50.0mL of sterile so

lution B, and 100.0mL of sterile solution C. Mix

thoroughly. Pour into sterile Petri dishes or aseptical

ly distribute into sterile tubes.

Use: For the cultivation and maintenance of a vari

ety of bacteria.