培養基配方

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M16 Agar

[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]

M16 Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................10.0g

Beef extract.........................................................5.0g

Pancreatic digest of soybean meal......................5.0g

Polypeptone ........................................................5.0g

Sodium acetate·3H2O..........................................3.0g

Yeast extract........................................................2.5g

Ascorbic acid ......................................................0.5g

Carbohydrate solution................................... 50.0mL

pH 7.2 ± 0.2 at 25°C

Carbohydrate Solution:

Composition per 50.0mL:

Carbohydrate.......................................................5.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 50.0mL. Lactose or glucose may be used.

Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 950.0mL. Mix thoroughly.

Gently heat and bring to boiling. Adjust pH to 7.2

with 2N NaOH. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Aseptically add

50.0mL of sterile carbohydrate solution. Mix thor

oughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the cultivation of lactobacilli from cheddar

cheese.

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