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Lysozyme Broth (BAM M90)

[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]
Lysozyme Broth
(BAM M90)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per 1010.0mL:

Nutrient broth..................................................... 1.0L
Lysozyme solution ........................................ 10.0mL
pH 6.9 ± 0.2 at 25°C
Nutrient Broth:
Composition per liter:
Pancreatic digest of gelatin.................................5.0g
Beef extract.........................................................3.0g
Source: Nutrient broth is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Nutrient Broth: Add compo
nents to distilled/deionized water and bring volume
to 1.0L. Mix thoroughly. Distribute into bottles in
99.0mL volumes. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 25°C.
Lysozyme Solution:
Composition per 100.0mL:
Lysozyme............................................................0.1g
Preparation of Lysozyme Solution: Add
lysozyme to distilled/deionized water and bring vol
ume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add 1.0mL of sterile
lysozyme solution to 99.0mL of cooled, sterile nutri
ent broth. Mix thoroughly. Aseptically distribute into
sterile tubes in 2.5mL volumes.
Use: For the cultivation and differentiation of Bacil
lus cereus in foods. Bacillus cereus is resistant to
lysozyme and will grow in this medium.
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