- 定做培養基/定制培養基
- 顆粒培養基
- 標準菌株生化鑒定試劑盒
- 預灌裝即用型成品培養基
- 2025年版中國藥典
- 促銷/特價商品
- 院感/疾控/體外診斷/采樣管
- 樣品采集與處理(均質)產品
- 按標準檢索培養基
- 模擬灌裝用培養基
- 干燥粉末培養基
- 培養基添加劑/補充劑
- 生化反應鑒定管
- 染色液等配套產品
- 對照培養基/標準品
- 實驗耗材與器具
- 生化試劑/化學試劑
- 菌種鑒定服務
培養基配方
您現在的位置： 網站首頁 >> 培養基配方
M17 Agar (LMG Medium 261)
[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]
M17 Agar
(LMG Medium 261)
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Disodium β-glycerophosphate..........................19.0g
Agar ..................................................................11.0g
Polypeptone ........................................................5.0g
Beef extract.........................................................5.0g
Papaic digest of soybean meal............................5.0g
Yeast extract........................................................2.5g
Ascorbic acid ......................................................0.5g
Lactose solution ............................................ 50.0mL
MgSO4·7H2O (1M solution) ........................... 1.0mL
pH 6.9 ± 0.2 at 25°C
Lactose Solution:
Composition per 100.0mL:
Lactose..............................................................10.0g
Preparation of Lactose Solution: Add lactose
to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Autoclave for 15 min at
15 psi pressure–121°C.
Preparation of Medium: Add components, ex
cept lactose solution, to distilled/deionized water and
bring volume to 950.0mL. Mix thoroughly. Gently
heat until boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add
50.0mL of sterile lactose solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the cultivation of Streptococcus thermophi
lus and for the cultivation and maintenance of strep
tococci and their bacteriophages. Also used for the
cultivation and maintenance of starter cultures for
cheese and yogurt manufacture as well as detecting
streptococcal mutants that are unable to ferment lac
tose.
