99精品国产成人综合_国产不卡精品视频男人的天堂_人妻av久久一区波多野结衣_亚洲乱码卡一卡二卡新区仙踪林_人妻无码av天堂二区网站_中文字幕精品av一区二区五区_国产精品国产高清国产av_亚洲欧美日韩国产综合点击进入_亚洲欧美日韩国产制服另类_亚洲精品成av人片天堂无码

<bdo id='c6klp'><sup id='c6klp'><div id='c6klp'><bdo id='c6klp'></bdo></div></sup></bdo>

展開

編號：
批號：

培養基配方

您現在的位置：網站首頁 >> 培養基配方

Lowenstein-Jensen Medium

[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per 1600.0mL:

Potato starch......................................................30.0g

Asparagine ..........................................................3.6g

KH2PO4...............................................................2.4g

Magnesium citrate...............................................0.6g

Malachite Green..................................................0.4g

MgSO4·7H2O ....................................................0.24g

Homogenized whole egg .................................. 1.0L

Glycerol ........................................................ 12.0mL

Source: This medium is available as a prepared me

dium from BD Diagnostic Systems and Oxoid.

Homogenized Whole Egg:

Composition per liter:

Whole eggs ...................................................... 18-24

Preparation of Homogenized Whole Egg:

Use fresh eggs, less than 1 week old. Scrub the shells

with soap. Let stand in a soap solution for 30 min.

Rinse in running water. Soak eggs in 70% ethanol for

15 min. Break the eggs into a sterile container. Ho

mogenize by shaking. Filter through four layers of

sterile cheesecloth into a sterile graduated cylinder.

Measure out 1.0L.

Preparation of Medium: Add glycerol to

600.0mL of distilled/deionized water. Mix thorough

ly. Add remaining components, except fresh egg

mixture. Mix thoroughly. Gently heat while stirring

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°C. Aseptically add 1.0L

of homogenized whole egg. Mix thoroughly. Distrib

ute into sterile screw-capped tubes. Place tubes in a

slanted position. Inspissate at 85°C (moist heat) for

45 min.

Use: For the cultivation and differentiation of Myco

bacterium species. Mycobacterium tuberculosis ap

pears as granular, rough, dry colonies. Mycobacterium

kansasii appears as smooth to rough photochromoge

nic colonies. Mycobacterium gordonae appears as

smooth yellow-orange colonies. Mycobacterium avi

um appears as smooth, colorless colonies. Mycobacte

rium smegmatis appears as wrinkled, creamy white

colonies. Also used for the cultivation and mainte

nance of Gordona species, Nocardia species, Rhodo

coccus species, and Tsukamurella paurometabolum.

在線客服

Lowenstein-Jensen Medium