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培養基配方
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Lowenstein-Gruft Medium
[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]
Lowenstein-Gruft Medium
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per 1600.0mL:
Potato starch......................................................30.0g
Asparagine ..........................................................3.6g
KH2PO4...............................................................2.4g
Magnesium citrate ..............................................0.6g
Malachite Green..................................................0.4g
MgSO4·7H2O....................................................0.24g
Nalidixic acid..................................................0.056g
Ribonucleic acid ............................................0.08mg
Homogenized whole egg .................................. 1.0L
Glycerol ........................................................ 12.0mL
Penicillin......................................................80,000U
Homogenized Whole Egg:
Composition per liter:
Whole eggs ......................................................18–24
Preparation of Homogenized Whole Egg:
Use fresh eggs, less than 1 week old. Scrub the shells
with soap. Let stand in a soap solution for 30 min.
Rinse in running water. Soak eggs in 70% ethanol for
15 min. Break the eggs into a sterile container. Ho
mogenize by shaking. Filter through four layers of
sterile cheesecloth into a sterile graduated cylinder.
Measure out 1.0L.
Preparation of Medium: Add glycerol to
600.0mL of distilled/deionized water. Mix thorough
ly. Add remaining components, except fresh egg
mixture. Mix thoroughly. Gently heat while stirring
and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add 1.0L
of homogenized whole egg. Mix thoroughly. Distrib
ute into sterile screw-capped tubes. Place tubes in a
slanted position. Inspissate at 85°C (moist heat) for
45 min.
Use: For the cultivation and differentiation of Myco
bacterium species. Mycobacterium tuberculosis ap-
pears as granular, rough, dry colonies. Mycobacterium
kansasii appears as smooth to rough photochromoge
nic colonies. Mycobacterium gordonae appears as
smooth yellow-orange colonies. Mycobacterium avi
um appears as smooth, colorless colonies. Mycobacte
rium smegmatis appears as wrinkled, creamy white
colonies.
