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Liver Infusion Sake Medium

[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]
Liver Infusion Sake Medium

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Liver, fresh......................................................400.0g
Agar ..................................................................15.0g
Sake............................................................. 400.0mL
Preparation of Medium: Finely mince liver and
add to 600.0mL of distilled/deionized water. Gently
heat and bring to boiling. Continue boiling for 5–10
min. Filter through Whatman #2 filter paper. To fil
trate, add agar and sake. Bring volume to 1.0L with
distilled/deionized water. Mix thoroughly. Gently
heat and bring to boiling. Distribute into tubes or
flasks. Autoclave for 25 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Lacto
bacillus fructivorans and Lactobacillus homohiochi.
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