培養基配方

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Liver Broth with NaCl

[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Beef liver, fresh...............................................453.0g

NaCl................................................................150.0g

Pancreatic digest of casein................................10.0g

K2HPO4...............................................................1.0g

Soluble starch......................................................1.0g

pH 7.6 ± 0.2 at 25°C

Preparation of Medium: Remove the fat from

fresh beef liver. Grind the liver. Add 1.0L of distilled/

deionized water. Gently heat and bring to boiling.

Continue boiling for 60 min. Adjust pH to 7.6. Filter

through cheesecloth. Reserve meat. To filtrate, add

pancreatic digest of casein, K2HPO4, NaCl, and sol

uble starch. Bring volume to 1.0L with distilled/

deionized water. Refilter solution. Add meat particles

to test tubes to a depth of approximately 2cm. Dis

tribute broth into tubes with meat particles in 15.0mL

volumes. Autoclave for 20 min at 15 psi pressure–

121°C. After inoculation, overlay each tube with

sterile, melted petroleum jelly.

Use: For the cultivation of obligate halophiles from

brined and dry-salted vegetables.