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Liver Veal Agar (BAM M83)

[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]

Liver Veal Agar

(BAM M83)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Veal, infusion from .........................................500.0g

Beef liver, infusion from...................................50.0g

Gelatin...............................................................20.0g

Proteose peptone...............................................20.0g

Agar ..................................................................15.0g

Soluble starch....................................................10.0g

Glucose ...............................................................5.0g

NaCl....................................................................5.0g

Casein .................................................................2.0g

NaNO3 ................................................................2.0g

Enzymatic digest of protein................................1.3g

Pancreatic digest of casein..................................1.3g

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Make sure that some

liver and veal particles are transferred to each tube.

Autoclave for 15 min at 15 psi pressure–121°C. Pour

into sterile Petri dishes or leave in tubes.

Use: For the cultivation of a variety of anaerobic or

ganisms.