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Litmus Milk

[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]
Litmus Milk

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Skim milk........................................................100.0g
Azolitmin ............................................................0.5g
Na2SO3................................................................0.5g
pH 6.5 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems and Oxoid.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 20 min
at 10 psi pressure–115°C.
Use: For the maintenance of lactic acid bacteria and
for the differentiation of several bacteria, especially
Clostridium species, based on their action on milk.
Bacteria that do not ferment carbohydrates, such as
Proteus vulgaris or Moraxella lacunata, show no
change in the litmus indicator. Bacteria that ferment
lactose or glucose with the production of gas, such as
Clostridium perfringens, turn the medium pink and
frothy. Bacteria that proteolytically degrade lactalbu
min turn the medium blue. Bacteria that coagulate
casein form a curd or clot. Bacteria that peptonize
casein, such as Pseudomonas aeruginosa, show a
dissolution of the clot.
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