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Litmus Lactose Agar with Crystal Violet (LLK Agar)

[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]
Litmus Lactose Agar with Crystal Violet
(LLK Agar)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................10.0g
Lactose..............................................................10.0g
Meat peptone.......................................................5.0g
Beef extract.........................................................3.0g
Litmus .................................................................1.0g
Crystal Violet ...................................................5.0mg
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dish
es or leave in tubes.
Use: For the maintenance of lactic acid bacteria and
for the differentiation of several bacteria on the basis
of lactose fermentation. Bacteria that ferment lactose
appear as red colonies. Bacteria that do not ferment
lactose appear as dark blue-purple colonies.
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