培養基配方

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Liver Broth

[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]

Liver Broth

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Extracted liver tissue, minced...........................30.0g

Infusion from fresh liver...................................23.0g

Peptone .............................................................10.0g

K2HPO4...............................................................1.0g

Agar overlay solution ................................. 200.0mL

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Agar Overlay Solution:

Composition per 200.0mL:

Agar, Oxoid No. 3...............................................4.0g

Preparation of Agar Overlay Solution: Add

agar to distilled/deionized water and bring volume to

200.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C.

Preparation of Medium: Add components, ex

cept agar, to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes to a depth of 50mm.

Make sure that some liver particles are transferred to

each tube. Autoclave for 20 min at 10 psi pressure–

115°C. After inoculation, aseptically overlayer the

broth with 2.0mL of sterile, cooled agar solution per

tube.

Use: For the isolation and cultivation of saccharolyt

ic or putrefactive mesophilic and thermophilic anaer

obic bacteria from foods.

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