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[所屬分類:培養基配方] [發布時間:2021-11-23] [發布人:網站管理員2] [閱讀次數:] [返回]
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山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per 1011.0mL:

Agar ..................................................................15.0g
Peptone................................................................5.0g
Yeast extract........................................................1.0g
Basal salts solution............................................. 1.0L
Lactose solution ................................................10.0g
Trace elements solution .................................. 1.0mL
Basal Salts Solution:
Composition per liter:
MgSO4 ................................................................0.5g
Phosphate solution ........................................ 20.0mL
(NH4)SO4 (36% solution) ............................... 5.0mL
Phosphate Solution:
Composition per liter:
K2HPO4.............................................................95.0g
NaH2PO4·2H2O.................................................78.0g
Preparation of Phosphate Solution: Add compo
nents to distilled/deionized water and bring volume
to 1.0L. Mix thoroughly.
Preparation of Basal Salts Medium: Add com
ponents to distilled/deionized water and bring vol
ume to 1.0L. Mix thoroughly. Adjust pH to 6.8-7.0.
Lactose Solution:
Composition per 10.0mL:
Lactose................................................................2.5g
Preparation of Lactose Solution: Add lactose
to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize. Warm to
50°–55°C.
Trace Elements Solution:
Composition per liter:
Disodium EDTA .................................................0.5g
FeSO4·7H2O........................................................0.2g
H3BO3 ...............................................................0.03g
CoCl2·6H2O ......................................................0.02g
ZnSO4·7H2O .....................................................0.01g
MnCl2·4H2O ....................................................3.0mg
Na2MoO4·2H2O ...............................................3.0mg
NiCl2·6H2O......................................................2.0mg
CaCl2·2H2O .....................................................1.0mg
Preparation of Trace Elements Solution: Add
components to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C.
Preparation of Medium: Combine components,
except lactose solution and trace elements solution.
Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°–55°C. Aseptically add 10.0mL of sterile lac
tose solution and 1.0mL of sterile trace elements so
lution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the cultivation of Bacillus species.
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