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培養基配方

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Imidazole Utilization Medium

[所屬分類:培養基配方] [發布時間:2021-11-23] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普(pu)生(sheng)物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Imidazole ............................................................5.0g

KH2PO4...............................................................0.5g

MgSO4·7H2O......................................................0.5g

CaCl2................................................................3.0mg

FeSO4·7H2O.....................................................3.0mg

Molybdenum solution..................................... 1.0mL

Trace element solution.................................... 1.0mL

pH 6.0 ± 0.2 at 25°C

Molybdenum Solution:

Composition per 18.0mL:

Na2MoO4·2H2O ...............................................0.5mg

Preparation of Molybdenum Solution: Add

components to distilled/deionized water and bring

volume to 18.0mL. Mix thoroughly. Filter sterilize.

Trace Element Solution:

Composition per 18.0mL:

H3BO3 ............................................................11.0mg

MnCl2·4H2O ....................................................7.0mg

Al2(SO4)3·18 H2O ..........................................1.94mg

Co(NO3)2·6H2O ...............................................1.0mg

CuSO4·5H2O....................................................1.0mg

NiSO4·6H2O.....................................................1.0mg

ZnSO4·H2O ....................................................0.62mg

KBr ..................................................................0.5mg

KI .....................................................................0.5mg

LiCl..................................................................0.5mg

SnCl2·2H2O......................................................0.5mg

Preparation of Trace Element Solution: Add

components to distilled/deionized water and bring

volume to 18.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept molybdenum solution and trace element solu

tion, to distilled/deionized water and bring volume to

998.0mL. Mix thoroughly. Distribute into tubes or

flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 25°C. Aseptically add 1.0mL of mo

lybdenum solution and 1.0mL of trace element solu

tion. Mix thoroughly. Adjust pH to 6.0 with

phosphoric acid. Mix thoroughly. Aseptically distrib

ute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Pseudo

monas species.

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Imidazole Utilization Medium