培養基配方

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Hydroxybenzoic Acid Medium

[所屬分類:培養基配方] [發布時間:2021-11-23] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公(gong)司培養(yang)基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g

K2HPO4·3H2O ..................................................4.25g

NH4Cl .................................................................2.0g

4-Hydroxybenzoic acid.......................................1.0g

NaH2PO4·H2O.....................................................1.0g

MgSO4·7H2O......................................................0.2g

Nitrilotriacetic acid .............................................0.1g

FeSO4·7H2O....................................................0.012g

MnSO4·H2O .....................................................3.0mg

ZnSO4·7H2O ....................................................3.0mg

CoSO4 ..............................................................1.0mg

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add 4-hydroxybenzoic

acid and nitrilotriacetic acid to approximately

600.0mL of distilled/deionized water. Adjust pH to

8.0 with concentrated NaOH. Add remaining compo

nents. Mix thoroughly. Readjust pH to 7.2. Bring vol

ume to 1.0L with distilled/deionized water.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Bacillus

species.