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培養基配方

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HL Agar

[所屬分類:培養基配方] [發布時間:2021-11-23] [發布人:網站管理員2] [閱讀次數:] [返回]

HL Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per plate:

Columbia agar base....................................... 10.0mL

Columbia blood top agar ................................ 5.0mL

pH 7.3 ± 0.2 at 25°C

Columbia Agar Base:

Composition per liter:

Agar ..................................................................13.5g

Pancreatic digest of casein................................12.0g

NaCl....................................................................5.0g

Peptic digest of animal tissue .............................5.0g

Beef extract.........................................................3.0g

Yeast extract........................................................3.0g

Cornstarch...........................................................1.0g

Preparation of Columbia Agar Base: Add

components to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly. Gently heat until

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C.

Columbia Blood Top Agar:

Composition per liter:

Agar ..................................................................13.5g

Pancreatic digest of casein................................12.0g

NaCl....................................................................5.0g

Peptic digest of animal tissue .............................5.0g

Beef extract.........................................................3.0g

Yeast extract........................................................3.0g

Cornstarch...........................................................1.0g

Horse blood, defibrinated ............................. 50.0mL

Preparation of Columbia Blood Top Agar:

Add components, except horse blood, to distilled/

deionized water and bring volume to 950.0mL. Mix

thoroughly. Gently heat until boiling. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile horse blood. Mix thoroughly.

Preparation of Medium: Pour cooled, sterile Co

lumbia agar base into sterile Petri dishes in 10.0mL

volumes. Allow agar to solidify. Pour 5.0mL of

cooled, sterile Columbia blood top agar over Colum

bia agar base that has solidified but is still warm.

Use: For the cultivation of Listeria monocytogenes.

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HL Agar