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HiCrome Salmonella Agar (Salmonella Agar, HiCrome)

[所屬分類:培養基配方] [發布時間:2021-11-23] [發布人:網站管理員2] [閱讀次數:] [返回]

HiCrome Salmonella Agar

(Salmonella Agar, HiCrome)

山東(dong)拓(tuo)普生物工程有限公(gong)司培養(yang)基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................13.0g

Peptic digest of animal tissue..............................6.0g

Chromogenic mixture .........................................5.4g

Yeast extract........................................................2.5g

Bile salt mixture..................................................1.0g

pH 7.7 ± 0.2 at 25°C

Source: This medium is available from Fluka, Sig

ma-Aldrich.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Mix to completely dissolve components.

Do not autoclave. Cool to 50°C. Pour into sterile Pet

ri dishes.

Use: A selective chromogenic medium used for the

isolation and differentiation of Salmonella species

from coliforms in food and water. E. coli and Salmo

nella are easily distinguishable due to the colony

characteristics. Salmonella give light purple colonies

with a halo. E. coli has a characteristic blue color.

Other organisms give colorless colonies. The charac

teristic light purple and blue color is due to the chro

mogenic mixture.