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Horie Arabinose Ethyl Violet Broth (HAEB)

[所屬分類:培養基配方] [發布時間:2021-11-23] [發布人:網站管理員2] [閱讀次數:] [返回]
Horie Arabinose Ethyl Violet Broth
(HAEB)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

NaCl..................................................................30.0g
Peptone................................................................5.0g
Beef extract.........................................................3.0g
Bromthymol Blue .............................................0.03g
Ethyl Violet......................................................1.0mg
Arabinose solution ...................................... 100.0mL
pH 9.0 ± 0.2 at 25°C
Arabinose Solution:
Composition per 100.0mL:
Arabinose............................................................5.0g
Preparation of Arabinose Solution: Add arabi
nose to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept arabinose solution, to distilled/deionized water
and bring volume to 900.0mL. Mix thoroughly. Gen
tly heat and bring to boiling. Adjust pH to 9.0. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add sterile arabinose solution.
Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.
Preparation of Medium: Dissolve all ingredi
ents, except arabinose, in 900.0mL of distilled water.
Adjust the pH to 9.0 ± 0.2 and sterilize at 121°C for
15 min. Cool and aseptically add 100.0mL of a filter
sterilized 5% arabinose solution in distilled water.
After mixing, aseptically dispense the medium into
sterile tubes or bottles.
Use: For the cultivation of Vibrio species from
foods.
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