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培養基配方

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HiCrome OGYE Agar Base (OGYE Agar Base HiCrome) (Oxytetracyclin Glucose Yeast Extract Agar HiCrome)

[所屬分類:培養基配方] [發布時間:2021-11-23] [發布人:網站管理員2] [閱讀次數:] [返回]

HiCrome OGYE Agar Base

(OGYE Agar Base HiCrome)

(Oxytetracyclin Glucose

Yeast Extract Agar HiCrome)

山東拓普(pu)生物工程有限公(gong)司培養基配方 //ljxinglong.com

Composition per liter:

Glucose ............................................................20.0g

Agar ..................................................................12.0g

Yeast extract .......................................................4.0g

Chromogenic mixture ........................................1.1g

Oxytetracycline selective supplement .......... 10.0mL

pH 7.0 ± 0.2 at 25°C

Oxytetracylcine Selective Supplement:

Composition per 10.0mL:

Oxytetracycline...................................................0.1g

Preparation of Oxytetracylcine Selective

Supplement: Add 0.1g oxytetracyline to 10.0mL

of distilled/deionized water. Mix thoroughly. Filter

sterilize.

Source: This medium is available from Fluka, Sig

ma-Aldrich.

Preparation of Medium: Add components, ex

cept oxytetracycline selective supplement, to dis

tilled/deionized water and bring volume to 990.0mL.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C. Aseptically add 10.0mL sterile

oxytetracylcine selective supplement. Mix thorough

ly. Pour into sterile Petri dishes.

Use: For the rapid isolation of yeasts and molds from

milk and milk products. Oxytetracycline makes the

medium more selective by inhibiting the growth of

lactobacilli. Aspergillus niger appears as light blue

colored colonies with black spores due to presence of

chromogenic mixture, Candida albicans shows

green colored colonies and Saccharomyces cerevisi

ae gives colorless colonies.

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