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HiCrome Rapid Coliform Broth (Coliform Rapid HiCrome Broth) (Rapid Coliform HiCrome Broth)

[所屬分類:培養基配方] [發布時間:2021-11-23] [發布人:網站管理員2] [閱讀次數:] [返回]

HiCrome Rapid Coliform Broth

(Coliform Rapid HiCrome Broth)

(Rapid Coliform HiCrome Broth)

山(shan)東拓普生物(wu)工(gong)程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Peptone, special ..................................................5.0g

NaCl....................................................................5.0g

Na2HPO4.............................................................2.7g

KH2PO4...............................................................2.0g

Sorbitol ...............................................................1.0g

Sodium lauryl sulfate..........................................0.1g

IPTG....................................................................0.1g

Chromogenic substrate .....................................0.08g

Fluorogenic substrate........................................0.05g

pH 6.8 ± 0.3 at 25°C

Source: This medium is available from Fluka, Sig

ma-Aldrich.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Pour into sterile Petri dishes.

Use: For the detection and conformation of Escher

ichia coli and coliforms on the basis of enzyme sub

strate reaction from water samples, using a combina

tion of chromogenic and fluorogenic substrate.