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HiCrome MS.O157 Agar with Tellurite (MS.O157 Agar with Tellurite, HiCrome)

[所屬分類:培養基配方] [發布時間:2021-11-23] [發布人:網站管理員2] [閱讀次數:] [返回]
HiCrome MS.O157 Agar
with Tellurite
(MS.O157 Agar
with Tellurite, HiCrome)

山東拓普生物工程有限公司 培(pei)養(yang)基配方 //ljxinglong.com

Composition 1000.25mL:

Agar ..................................................................12.0g
Peptone, special ................................................10.0g
Sorbitol................................................................4.0g
Bile salt mixture..................................................1.0g
Chromogenic mixture .....................................0.731g
Tellurite solution ........................................... 0.25mL
pH 6.8 ± 0.2 at 25°C
Tellurite Solution:
Composition per 10.0mL:
K2TeO3 ................................................................0.1g
Preparation of Tellurite Solution: Add compo
nents to 10.0mL of distilled/deionized water. Mix
thoroughly. Filter sterilize.
Caution: Potassium tellurite is toxic.
Source: This medium is available from Fluka, Sig
ma-Aldrich.
Preparation of Medium: Add components, ex
cept tellurite solution, to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Gently heat
while stirring and bring to boiling. Do not autoclave.
Cool to 45°C. Aseptically add 0.25mL sterile tellurite
solution. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the simultaneous detection of Escherichia
coli, Escherichia coli O157:H7, and coliforms in wa
ter and food samples. Escherichia coli O157:H7 gives
colorless colonies because of non-fermentation of sor
bitol and absence of β-glucuronidase activity, whereas
other strains of Escherichia coli having β-glucu
ronidase activity and fermenting sorbitol appear as
steel blue colored colonies. Addition of tellurite makes
the medium much more specific and selective.
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