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HiCrome MS.O157 Agar (MS.O157 Agar HiCrome)

[所屬分類:培養基配方] [發布時間:2021-11-23] [發布人:網站管理員2] [閱讀次數:] [返回]
HiCrome MS.O157 Agar
(MS.O157 Agar HiCrome)

山(shan)東(dong)拓(tuo)普生物工程有限公司 培養(yang)基(ji)配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................12.0g
Peptone, special ................................................10.0g
Sorbitol ...............................................................4.0g
Bile salt mixture..................................................1.0g
Chromogenic mixture .....................................0.731g
pH 6.8 ± 0.2 at 25°C
Source: This medium is available from Fluka, Sig
ma-Aldrich.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring and bring
to boiling. Do not autoclave. Cool to 50°C. Pour into
sterile Petri dishes.
Use: For the simultaneous detection of Escherichia
coli, Escherichia coli O157:H7, and coliforms in wa
ter and food samples. Escherichia coli O157 gives
colorless colonies because of non-fermentation of
sorbitol and absence of β-glucuronidase activity,
whereas other strains of Escherichia coli having β-
glucuronidase activity and fermenting sorbitol ap
pear as steel blue colored colonies. Some non-Es
cherichia coli O157 may have some colony color.
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