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Fletcher Medium

[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]
Fletcher Medium

山東拓普生(sheng)物工程有限公(gong)司 培養基配(pei)方 //ljxinglong.com

Composition per liter:

Agar ...................................................................1.5g
NaCl...................................................................0.5g
Peptone...............................................................0.3g
Beef extract........................................................0.2g
Rabbit serum................................................ 50.0mL
pH 7.9 ± 0.1 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components, ex
cept rabbit serum, to distilled/deionized water and
bring volume to 950.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 50°–55°C. Aseptically
add 50.0mL of sterile rabbit serum. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the isolation, cultivation, and maintenance
of cultures of Leptospira species.
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