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Fluid Thioglycolate Agar with Calcium Carbonate

[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]

Fluid Thioglycolate Agar

with Calcium Carbonate

山東拓普生物工程有限公司培養基(ji)配方 //ljxinglong.com

Composition per liter:

Agar .................................................................75.0g

Pancreatic digest of casein...............................15.0g

CaCO3 ..............................................................10.0g

Glucose ..............................................................5.0g

Yeast extract.......................................................5.0g

NaCl...................................................................2.5g

L-Cystine ............................................................0.5g

Sodium thioglycolate .........................................0.5g

Resazurin .......................................................0.001g

pH 7.1 ± 0.2 at 25°C

Preparation of Medium: Add components, ex

cept CaCO3, to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly. Gently heat and

bring to boiling. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Sterilize

CaCO3 by autoclaving for 15 min at 15 psi pressure–

121°C. Aseptically add sterile CaCO3 to sterile tubes

or plates—0.1g of sterile CaCO3 per 10.0mL of me

dium to be added. Pour medium into sterile Petri

dishes or distribute into sterile tubes.

Use: For the rapid cultivation of anaerobic bacteria.

For the cultivation and maintenance of Campylo

bacter fetus, Campylobacter jejuni, and Streptococ

cus species.