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Fletcher Medium with Fluorouracil (Fluorouracil Leptospira Medium)
[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]
Fletcher Medium with Fluorouracil
(Fluorouracil Leptospira Medium)
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Agar ...................................................................1.5g
NaCl................................................................... 0.5g
Peptone .............................................................. 0.3g
Beef extract........................................................ 0.2g
Rabbit serum................................................ 50.0mL
Fluorouracil solution.................................... 20.0mL
pH 7.9 ± 0.1 at 25°C
Fluorouracil Solution:
Composition per 100.0mL:
Fluorouracil...................................................... 10.0g
Preparation of Fluorouracil Solution: Add
fluorouracil to 50.0mL of distilled/deionized water.
Add 1.0mL of 2N NaOH and bring volume to
100.0mL. Gently heat to 56°C for 2 hr. Adjust pH to
7.4–7.6 with NaOH. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept rabbit serum and fluorouracil solution, to dis
tilled/deionized water and bring volume to 930.0mL.
Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°–55°C. Aseptically add 80.0mL of sterile rab
bit serum. Mix thoroughly. Aseptically distribute into
sterile tubes or flasks. Immediately prior to use, add
0.1mL of fluorouracil solution per 5.0mL of medium.
Use: For the isolation, cultivation, and maintenance
of cultures of Leptospira species.
