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培養基配方

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ETGPA (Egg Tellurite Glycine Pyruvate Agar)

[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]

ETGPA

(Egg Tellurite Glycine Pyruvate Agar)

山東拓(tuo)普生(sheng)物(wu)工程有限(xian)公(gong)司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................17.0g

Glycine..............................................................12.0g

Sodium pyruvate...............................................10.0g

Pancreatic digest of casein................................10.0g

Beef extract.........................................................5.0g

LiCl .....................................................................5.0g

Yeast extract........................................................1.0g

Egg yolk emulsion ........................................ 50.0mL

K2TeO3 solution............................................ 10.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Egg Yolk Emulsion:

Composition:

Chicken egg yolks..................................................11

Whole chicken egg ..................................................1

Preparation of Egg Yolk Emulsion: Soak egg

with 1:100 dilution of saturated mercuric chloride so

lution for 1 min. Crack eggs and separate yolks from

whites. Mix egg yolks with 1 chicken egg.

K2TeO3 Solution:

Composition per 100.0mL:

K2TeO3................................................................1.0g

Preparation of K2TeO3 Solution: Add K2TeO3

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Caution: Potassium tellurite is toxic.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to

940.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Add 10.0mL of sterile 1%

tellurite solution and 50.0mL of sterile egg yolk

emulsion. If desired, add sulfamethazine to a final

concentration of 50.0mg/mL. Mix thoroughly but

gently and pour into sterile Petri dishes.

Use: For the selective isolation and enumeration of

coagulase-positive staphylococci from food. For the

differentiation and identification of staphylococci on

the basis of their ability to clear egg yolk. Addition of

sulfamethazine inhibits the growth of Proteus. Gray

black colonies surrounded by a clear zone are diag

nostic for Staphylococcus aureus.

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ETGPA (Egg Tellurite Glycine Pyruvate Agar)