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培養基配方

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Esculin Mannitol Agar

[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................13.5g

Polypeptone ......................................................10.0g

D-Mannitol ........................................................10.0g

Pancreatic digest of casein..................................5.0g

Yeast extract........................................................5.0g

NaCl....................................................................5.0g

Heart peptone......................................................3.0g

Cornstarch...........................................................1.0g

Esculin ................................................................1.0g

Ferric ammonium citrate.....................................0.5g

Phenol Red......................................................0.025g

Nalidixic acid solution.................................. 10.0mL

Colistin solution............................................ 10.0mL

pH 7.3 ± 0.2 at 25°C

Nalidixic Acid Solution:

Composition per 10.0mL:

Nalidixic acid..................................................0.015g

Preparation of Nalidixic Acid Solution: Add

nalidixic acid to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Colistin Solution:

Composition per 10.0mL:

Colistin..............................................................0.01g

Preparation of Colistin Solution: Add colistin

to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept nalidixic acid solution and colistin solution, to

distilled/deionized water and bring volume to

980.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add sterile na

lidixic acid solution and colistin solution. Mix thor

oughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the selective isolation, cultivation, and dif

ferentiation of Staphylococcus aureus and group D

streptococci based on mannitol fermentation and hy

drolysis of esculin. Bacteria that ferment mannitol

appear as yellow colonies surrounded by a yellow

zone. Bacteria that hydrolyze esculin appear as dark

brown to black colonies surrounded by a dark brown

to black zone.

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Esculin Mannitol Agar