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Ethyl Violet Azide Broth (EVA Broth)

[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]
Ethyl Violet Azide Broth
(EVA Broth)

山東(dong)拓普生(sheng)物(wu)工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................13.5g
Yeast extract........................................................6.5g
Glucose ...............................................................5.0g
NaCl....................................................................5.0g
K2HPO4...............................................................2.7g
KH2PO4...............................................................2.7g
NaN3 ...................................................................0.4g
Ethyl Violet....................................................0.83mg
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes in 10.0mL volumes. Autoclave
for 15 min at 15 psi pressure–121°C.
Caution: Sodium azide is toxic. Azides also react
with metals and disposal must be highly diluted.
Use: For the isolation, cultivation, and enumeration
of enterococci. Fecal enterococci turn the medium
turbid with a purple sediment on the bottom of the
tube.
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