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Esculin Agar, Modified CDC (BAM M53)

[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]
Esculin Agar, Modified CDC
(BAM M53)

山東拓普(pu)生物工程有限公司(si) 培(pei)養基(ji)配方 //ljxinglong.com

Composition per liter:

Heart muscle, infusion from ...........................375.0g
Agar ..................................................................15.0g
Thiotone............................................................10.0g
NaCl....................................................................5.0g
Esculin ................................................................1.0g
Ferric citrate........................................................0.5g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Cool to 55°C. Adjust pH to 7.0. Distribute into tubes
or leave in flask. Autoclave for 20 min at 15 psi pres
sure–121°C. Pour into sterile Petri dishes or leave in
tubes. Allow tubes to cool in inclined position to pro
duce slants.
Use: For the differentiation of Enterobacter spp.
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