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培養基配方
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Esculin Broth
[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]
Esculin Broth
山東(dong)拓(tuo)普生物工程有限(xian)公(gong)司(si) 培養基配(pei)方 //ljxinglong.com
Composition per liter:
Beef heart, solids from infusion......................500.0g
Tryptose ............................................................10.0g
NaCl....................................................................5.0g
Agar ....................................................................1.0g
Esculin ................................................................1.0g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into screw-capped tubes in 7.0mL vol
umes. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation and differentiation of bacte
ria based on their ability to hydrolyze esculin. Bacte
ria that hydrolyze esculin turn the medium brown
black to black.
