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Esculin Agar

[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]
Esculin Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g
Pancreatic digest of casein................................13.0g
NaCl....................................................................5.0g
Yeast extract........................................................5.0g
Heart muscle, solids from infusion .....................2.0g
Esculin ................................................................1.0g
Ferric citrate........................................................0.5g
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into screw-capped tubes in 3.0mL vol
umes. Autoclave for 15 min at 15 psi pressure–
121°C. Allow tubes to cool in a slanted position.
Use: For the cultivation and differentiation of bacte
ria based on their ability to hydrolyze esculin and
produce H2S. Bacteria that hydrolyze esculin appear
as colonies surrounded by a reddish-brown to dark
brown zone. Bacteria that produce H2S appear as
black colonies.
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