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Enterococcus Agar (m-Enterococcus Agar) (Azide Agar)

[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]

Enterococcus Agar

(m-Enterococcus Agar)

(Azide Agar)

山東拓普生物工(gong)程有限公司培(pei)養基(ji)配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................15.0g

Agar ..................................................................10.0g

Papaic digest of soybean meal............................5.0g

Yeast extract........................................................5.0g

KH2PO4...............................................................4.0g

Glucose ...............................................................2.0g

NaN3 ...................................................................0.4g

Triphenyltetrazolium chloride ............................0.1g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Caution: Sodium azide is toxic. Azides also react

with metals and disposal must be highly diluted.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Cool to

45°–50°C. Do not autoclave. Pour into sterile Petri

dishes.

Use: For the isolation, cultivation, and enumeration

of entercocci in water, sewage and feces by the mem

brane filter method. For the direct plating of speci

mens for the detection and enumeration of fecal

streptococci.

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