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Demi-Fraser Broth

[所屬分類:培養基配方] [發布時間:2021-11-15] [發布人:網站管理員2] [閱讀次數:] [返回]
Demi-Fraser Broth

山東拓普生物(wu)工程有限公司 培養基(ji)配方 //ljxinglong.com

Composition per liter:

NaCl..................................................................20.0g
Tryptose ............................................................10.0g
Na2HPO4 .............................................................9.6g
Beef extract.........................................................5.0g
Yeast extract........................................................5.0g
LiCl .....................................................................3.0g
KH2PO4.............................................................1.35g
Esculin ................................................................1.0g
Acriflavin·HCl ...............................................12.5mg
Nalidixic acid.................................................10.0mg
Ferric ammonium citrate supplement ........... 10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed pow
der and supplement from BD Diagnostic Systems.
Ferric Ammonium Citrate Supplement:
Composition per 10.0mL:
Ferric ammonium citrate.....................................0.5g
Preparation of Ferric Ammonium Citrate
Supplement: Add ferric ammonium citrate to dis
tilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except
ferric ammonium citrate supplement, to distilled/deion
ized water and bring volume to 990.0mL. Mix thor
oughly. Autoclave for 15 min at 15 psi pressure–121°C.
Aseptically add 10.0mL of sterile ferric ammonium cit
rate supplement. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation of Listeria species from
foods.
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