培養基配方

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Deoxycholate Agar

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Deoxycholate Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................16.0g

Lactose..............................................................10.0g

NaCl....................................................................5.0g

Pancreatic digest of casein..................................5.0g

Peptic digest of animal tissue..............................5.0g

K2HPO4...............................................................2.0g

Ferric citrate........................................................1.0g

Sodium citrate.....................................................1.0g

Sodium deoxycholate..........................................1.0g

Neutral Red.....................................................0.033g

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed pow

der from BD Diagnostic Systems.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Do not au

toclave. Cool to 45°–50°C. Pour into sterile Petri dishes.

Use: For the selective isolation, cultivation, enumera

tion, and differentiation of Gram-negative enteric mi

croorganisms. Escherichia coli appears as large, flat,

rose-red colonies. Enterobacter and Klebsiella species

appear as large, mucoid, pale colonies with a pink cen

ter. Proteus and Salmonella species appear as large,

colorless to tan colonies. Shigella species appear as

colorless to pink colonies. Pseudomonas species ap

pear as irregular colorless to brown colonies.