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Decarboxylase Medium, Ornithine Modified

[所屬分類:培養基配方] [發布時間:2021-11-15] [發布人:網站管理員2] [閱讀次數:] [返回]
Decarboxylase Medium,
Ornithine Modified

山東拓普(pu)生物工程(cheng)有限公司 培養基配方 //ljxinglong.com

Composition per liter:

L-Ornithine ........................................................10.0g
Meat peptone ......................................................5.0g
Yeast extract........................................................3.0g
Bromcresol Purple solution ............................ 5.0mL
pH 5.5 ± 0.2 at 25°C
Bromcresol Purple Solution:
Composition per 100.0mL:
Bromcresol Purple ..............................................0.2g
Ethanol.......................................................... 50.0mL
Preparation of Bromcresol Purple Solution:
Add Bromcresol Purple to ethanol. Mix thoroughly.
Bring volume to 100.0mL with distilled/deionized wa
ter. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat until dissolved. Adjust pH to
5.5 with HCl or NaOH. Distribute into screw-capped
tubes. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and differentiation of bacte
ria based on their ability to decarboxylate ornithine.
Bacteria that decarboxylate ornithine turn the medi
um turbid purple.
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