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Decarboxylase Medium Base, Falkow

[所屬分類:培養基配方] [發布時間:2021-11-15] [發布人:網站管理員2] [閱讀次數:] [返回]
Decarboxylase Medium Base, Falkow

山東拓普(pu)生物工(gong)程有(you)限公司 培養基配方 //ljxinglong.com

Composition per liter:

Amino acid..........................................................5.0g
Peptone ...............................................................5.0g
Yeast extract........................................................3.0g
Glucose ...............................................................1.0g
Bromcresol Purple ............................................0.02g
Mineral oil .................................................. 200.0mL
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed pow
der from BD Diagnostic Systems.
Preparation of Medium: Add components, except
mineral oil, to distilled/deionized water and bring vol
ume to 1.0L. For amino acid, use L-arginine, L-lysine, or
L-ornithine. Mix thoroughly. Distribute into screw
capped tubes in 5.0mL volumes. Autoclave medium
and mineral oil separately for 15 min at 15 psi pressure–
121°C. After inoculation, overlay medium with 1.0mL
of sterile mineral oil per tube.
Use: For the cultivation and differentiation of bacte
ria based on their ability to decarboxylate the amino
acid. Bacteria that decarboxylate arginine, lysine, or
ornithine turn the medium turbid purple.
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