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Congo Red Agar (CR Agar)

[所屬分類:培養基配方] [發布時間:2021-11-12] [發布人:網站管理員2] [閱讀次數:] [返回]
Congo Red Agar
(CR Agar)

山東拓普生物(wu)工程有限公(gong)司 培養(yang)基配方 //ljxinglong.com

Composition per liter:

GC agar base............................................... 890.0mL
Hemoglobin solution .................................. 100.0mL
Supplement solution ..................................... 10.0mL
Congo Red (0.01% solution) .......................... 0.1mL
pH 7.2 ± 0.2 at 25°C
GC Agar Base:
Composition per 890.0mL:
Agar ..................................................................10.0g
Pancreatic digest of casein..................................7.5g
Peptic digest of animal tissue .............................7.5g
NaCl....................................................................5.0g
K2HPO4...............................................................4.0g
Cornstarch...........................................................1.0g
KH2PO4...............................................................1.0g
Preparation of GC Agar Base: Add components
to distilled/deionized water and bring volume to
890.0mL. Mix thoroughly. Gently heat until boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Hemoglobin Solution:
Composition per 100.0mL:
Hemoglobin ........................................................2.0g
Preparation of Hemoglobin Solution: Add he
moglobin to distilled/deionized water and bring vol
ume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°C.
Congo Red Solution:
Composition per 100.0mL:
Congo Red ........................................................0.01g
Preparation of Congo Red Solution: Add Congo
Red to 100.0mL of distilled/deionized water. Mix thor
oughly. Autoclave for 15 min at 15 psi pressure–
121°C.
Supplement Solution:
Composition per liter:
Glucose ...........................................................100.0g
L-Cysteine·HCl..................................................25.9g
L-Glutamine.......................................................10.0g
L-Cystine .............................................................1.1g
Adenine...............................................................1.0g
Nicotinamide adenine dinucleotide ..................0.25g
Vitamin B12 .........................................................0.1g
Thiamine pyrophosphate.....................................0.1g
Guanine·HCl .....................................................0.03g
Fe(NO3)3·6H2O.................................................0.02g
p-Aminobenzoic acid......................................0.013g
Thiamine·HCl ..................................................3.0mg
Preparation of Supplement Solution: Add
components to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Filter sterilize.
Source: The supplement solution IsoVitaleX® en
richment is available from BD Diagnostic Systems.
This enrichment may be replaced by supplement VX
from BD Diagnostic Systems.
Preparation of Medium: To 890.0mL of sterile,
cooled GC agar base, aseptically add 100.0mL of ster
ile, cooled hemoglobin solution, 10.0mL of sterile
supplement solution, and 0.1mL of sterile Congo Red
solution. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the isolation and differentiation of virulent
and avirulent strains of Shigella, Vibrio cholerae, and
Escherichia coli.
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