培養基配方

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Colonization Medium

[所屬分類:培養基配方] [發布時間:2021-11-12] [發布人:網站管理員2] [閱讀次數:] [返回]

Colonization Medium

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per 700.0mL:

Mannose..............................................................1.0g

Pancreatic digest of gelatin.................................0.2g

Brain heart, solids from infusion ......................0.08g

Peptic digest of animal tissue............................0.08g

NaCl..................................................................0.07g

Glucose .............................................................0.04g

Na2HPO4 ...........................................................0.03g

Bile salts No. 3....................................................0.1g

Dulbecco’s phosphate-buffered saline ........ 700.0mL

pH 7.4 ± 0.2 at 25°C

Dulbecco’s Phosphate-Buffered Saline:

Composition per liter:

NaCl....................................................................8.0g

Na2HPO4·7H2O.................................................2.16g

KCl......................................................................0.2g

KH2PO4...............................................................0.2g

CaCl2...................................................................0.1g

MnCl2·6H2O .......................................................0.1g

Preparation of Dulbecco’s Phosphate-Buff

ered Saline: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Combine components.

Mix thoroughly. Filter sterilize. Aseptically distrib

ute into sterile tubes or flasks.

Use: For the differentiation of enterotoxigenic Es

cherichia coli from foods based on the HeLa cell test

for colonization.