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培養基配方

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Congo Red Agar (CR Agar)

[所屬分類:培養基配方] [發布時間:2021-11-12] [發布人:網站管理員2] [閱讀次數:] [返回]

Congo Red Agar

(CR Agar)

山(shan)東拓普生物工程有限公(gong)司(si) 培養基配方(fang) //ljxinglong.com

Composition per liter:

Soybean-casein digest agar......................... 890.0mL

Hemoglobin solution................................... 100.0mL

Supplement solution ..................................... 10.0mL

Congo Red (0.01% solution) .......................... 0.1mL

pH 7.3 ± 0.2 at 25°C

Soybean-Casein Digest Agar:

Composition per 890.0mL:

Pancreatic digest of casein................................17.0g

Agar ..................................................................15.0g

NaCl....................................................................5.0g

Papaic digest of soybean meal............................3.0g

Glucose ...............................................................2.5g

K2HPO4...............................................................2.5g

Preparation of Soybean-Casein Digest Agar:

Add components to distilled/deionized water and

bring volume to 890.0mL. Mix thoroughly. Gently

heat until boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C.

Hemoglobin Solution:

Composition per 100.0mL:

Hemoglobin ........................................................2.0g

Preparation of Hemoglobin Solution: Add he

moglobin to distilled/deionized water and bring vol

ume to 100.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°C.

Congo Red Solution:

Composition per 100.0mL:

Congo Red ........................................................0.01g

Preparation of Congo Red Solution: Add Con

go Red to 100.0mL of distilled/deionized water. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C.

Supplement Solution:

Composition per liter:

Glucose ...........................................................100.0g

L-Cysteine·HCl..................................................25.9g

L-Glutamine ......................................................10.0g

L-Cystine .............................................................1.1g

Adenine...............................................................1.0g

Nicotinamide adenine dinucleotide ..................0.25g

Vitamin B12 .........................................................0.1g

Thiamine pyrophosphate ....................................0.1g

Guanine·HCl .....................................................0.03g

Fe(NO3)3·6H2O.................................................0.02g

p-Aminobenzoic acid......................................0.013g

Thiamine·HCl ..................................................3.0mg

Preparation of Supplement Solution: Add

components to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly. Filter sterilize.

Preparation of Medium: To 890.0mL of sterile,

cooled soybean-casein digest agar, aseptically add

100.0mL of sterile, cooled hemoglobin solution,

10.0mL of sterile supplement solution, and 0.1mL of

sterile Congo Red solution. Mix thoroughly. Pour into

sterile Petri dishes.

Source: The supplement solution IsoVitaleX en

richment is available from BD Diagnostic Systems.

This enrichment may be replaced by supplement VX

from BD Diagnostic Systems.

Use: For the isolation and differentiation of virulent

and avirulent strains of Shigella, Vibrio cholerae, and

Escherichia coli. Used for the detection and differen

tiation of “iron-responsive” avirulent mutants.

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Congo Red Agar (CR Agar)