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Coliform Medium, Modified (MCM)

[所屬分類:培養基配方] [發布時間:2021-11-12] [發布人:網站管理員2] [閱讀次數:] [返回]
Coliform Medium, Modified
(MCM)

山東拓普(pu)生物工(gong)程(cheng)有限公司 培(pei)養基(ji)配方(fang) //ljxinglong.com

Composition per liter:

Lactose..............................................................20.0g
Tris(hydroxymethyl)aminomethane buffer.......12.1g
Proteose peptone No. 3 .....................................10.0g
Yeast extract........................................................6.0g
Bile salts No. 3....................................................1.0g
Sodium lauryl sulfate..........................................1.0g
Sodium deoxycholate..........................................0.1g
Bromcresol Purple solution .......................... 10.0mL
pH 6.8 ± 0.2 at 25°C
Bromcresol Purple Solution:
Composition per 100.0mL:
Bromcresol Purple ............................................0.35g
NaOH (0.1N solution)..................................... 2.0mL
Preparation of Bromcresol Purple Solution:
Combine Bromcresol Purple and NaOH solution.
Mix thoroughly. Bring volume to 100.0mL with dis
tilled/deionized water. Filter sterilize.
Preparation of Medium: Add components, ex
cept Bromcresol Purple solution, to distilled/deion
ized water and bring volume to 990.0mL. Mix
thoroughly. Add 10.0mL of Bromcresol Purple solu
tion. Mix thoroughly. Adjust pH to 6.8 with 1N
NH4OH. Distribute into flasks in 95.0mL volumes.
Autoclave for 25 min at 15 psi pressure–121°C.
Use: For the isolation and cultivation of coliform mi
croorganisms from yogurt.
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