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Clostridium Selective Agar (Clostrisel Agar)

[所屬分類:培養基配方] [發布時間:2021-11-12] [發布人:網站管理員2] [閱讀次數:] [返回]
Clostridium Selective Agar
(Clostrisel Agar)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................17.0g
Agar ..................................................................14.0g
Glucose ...............................................................6.0g
Papaic digest of soybean meal............................3.0g
NaCl....................................................................2.5g
Sodium thioglycolate..........................................1.8g
Sodium formaldehyde sulfoxylate......................1.0g
L-Cystine ...........................................................0.25g
NaN3 .................................................................0.15g
Neomycin sulfate..............................................0.15g
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring and bring
to boiling. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–118°C. Pour into sterile
Petri dishes or leave in tubes.
Caution: Sodium azide is toxic. Azides also react
with metals and disposal must be highly diluted.
Use: For the selective isolation of pathogenic
Clostridium species from specimens containing
mixed flora.
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