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Coagulase Agar Base

[所屬分類:培養基配方] [發布時間:2021-11-12] [發布人:網站管理員2] [閱讀次數:] [返回]

Coagulase Agar Base

山東拓(tuo)普(pu)生物工程有限公司培養(yang)基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................25.0g

Brain heart infusion ..........................................10.5g

Pancreatic digest of casein................................10.5g

D-Mannitol ........................................................10.0g

Brain heart infusion ............................................5.0g

NaCl....................................................................3.5g

Papaic digest of soybean meal............................3.5g

Bromcresol Purple ............................................0.02g

Rabbit plasma ............................................. 100.0mL

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components, ex

cept rabbit plasma, to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Gently heat,

while stirring, until boiling. Distribute into tubes or

flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Add rabbit plasma to a fi

nal concentration of 7–15%. Mix thoroughly. Pour

into sterile Petri dishes in 18.0mL volume per plate.

Use: For the cultivation and differentiation of Sta

phylococcus aureus from other Staphylococcus spe

cies based on coagulase production.