<dd id='J0ylP'></dd>

培養基配方

您現在的位置：網站首頁 >> 培養基配方

Clostridium perfringens Agar, OPSP (Perfringens Agar, OPSP)

[所屬分類:培養基配方] [發布時間:2021-11-12] [發布人:網站管理員2] [閱讀次數:] [返回]

Clostridium perfringens Agar, OPSP

(Perfringens Agar, OPSP)

山(shan)東(dong)拓普生物工程有限公司培養基配方(fang) //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................15.0g

Agar ..................................................................10.0g

Liver extract........................................................7.0g

Papaic digest of soybean meal............................5.0g

Yeast extract........................................................5.0g

Tris(hydroxymethyl)aminomethane buffer.........1.5g

Ferric ammonium citrate.....................................1.0g

Na2S2O5 ..............................................................1.0g

Antibiotic inhibitor ....................................... 10.0mL

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Antibiotic Inhibitor:

Composition per 10.0mL:

Sodium sulfadiazine............................................0.1g

Oleandomycin phosphate.................................0.5mg

Polymyxin B ................................................10,000U

Preparation of Antibiotic Inhibitor: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except

antibiotic inhibitor, to distilled/deionized water and

bring volume to 990.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add sterile antibiotic inhibitor. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the presumptive identification and enumer

ation of Clostridium perfringens in foods.

<bdo id='u6TuQ'><sup id='Db8G8'><div id='vUVal'><bdo id='wDX6a'></bdo></div></sup></bdo>