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Cholera Medium TCBS

[所屬分類:培養基配方] [發布時間:2021-11-11] [發布人:網站管理員2] [閱讀次數:] [返回]
Cholera Medium TCBS

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Sucrose..............................................................20.0g
Agar ..................................................................14.0g
Peptone..............................................................10.0g
NaCl..................................................................10.0g
Sodium citrate...................................................10.0g
Na2S2O3·5H2O ..................................................10.0g
Ox bile.................................................................8.0g
Yeast extract........................................................5.0g
Ferric citrate........................................................1.0g
Bromthymol Blue .............................................0.04g
Thymol Blue .....................................................0.04g
pH 8.6 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from Oxoid.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling. Do
not autoclave. Pour into sterile Petri dishes. Dry agar
plates before using.
Use: For the growth of Vibrio cholerae, Vibrio para
haemolyticus, and other Vibrio species.
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