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Chopped Liver Broth (BAM M38)

[所屬分類:培養基配方] [發布時間:2021-11-11] [發布人:網站管理員2] [閱讀次數:] [返回]
Chopped Liver Broth
(BAM M38)

山東拓普(pu)生(sheng)物工(gong)程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Fresh beef liver ...............................................500.0g
Peptone..............................................................10.0g
K2HPO4 ..............................................................1.0g
Soluble starch......................................................1.0g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Grind fresh beef liver.
Add to 1.0L of distilled/deionized water. Gently heat
and bring to boiling. Continue boiling for 60 min.
Cool to 25°C. Adjust pH to 7.0. Gently heat and bring
to boiling. Continue boiling for 10 min. Filter
through cheesecloth. Press out excess liquid. Save
chopped liver particles. To filtrate, add remaining
components. Bring volume to 1.0L with distilled/
deionized water. Adjust pH to 7.0. Filter through
Whatman #1 filter paper. Add chopped liver particles
to test tubes to a depth of 1.2–2.5 cm. Add 10.0mL of
broth to each tube. Autoclave for 15 min at 15 psi
pressure–121°C.
Use: For the isolation and cultivation of Clostridium
botulinum, Clostridium perfringens, and other anaer
obic bacteria from foods.
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