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CHO Medium Base (Carbohydrate Medium Base)

[所屬分類:培養基配方] [發布時間:2021-11-11] [發布人:網站管理員2] [閱讀次數:] [返回]
CHO Medium Base
(Carbohydrate Medium Base)

山東拓普生物工程有限(xian)公(gong)司 培養基(ji)配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................15.0g
Yeast extract........................................................7.0g
NaCl....................................................................2.5g
Agar ..................................................................0.75g
Sodium thioglycolate..........................................0.5g
L-Cystine ...........................................................0.25g
Ascorbic acid ......................................................0.1g
Bromthymol Blue .............................................0.01g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C.
Use: Used as a basal medium to which carbohy
drates are added for fermentation studies of anaero
bic bacteria. Generally, 6.25mL of a 10% filter-ster
ilized solution of carbohydrate is added to the sterile
basal medium.
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