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Casamino Acids Yeast Extract Salts Broth, Gorbach (CA YE Broth) (BAM M34)

[所屬分類:培養基配方] [發布時間:2021-11-11] [發布人:網站管理員2] [閱讀次數:] [返回]
Casamino Acids Yeast
Extract Salts Broth, Gorbach
(CA YE Broth)
(BAM M34)

山東拓(tuo)普生物工(gong)程有限公司(si) 培養基配方 //ljxinglong.com

Composition per liter:

Casamino acids .................................................20.0g
K2HPO4 ............................................................8.71g
Yeast extract........................................................6.0g
NaCl....................................................................2.5g
Trace salts solution ......................................... 1.0mL
pH 8.5 ± 0.2 at 25°C
Trace Salts Solution:
Composition per liter:
MgSO4·7H2O ....................................................50.0g
MnCl2· 4H2O ......................................................5.0g
FeCl2 ...................................................................5.0g
Preparation of Trace Salts Solution: Add com
ponents to distilled/deionized water and bring vol
ume to 1.0L. Add sufficient 0.1N H2SO4 to dissolve
components. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept trace salts solution, to distilled/deionized water
and bring volume to 999.0mL. Mix thoroughly. Ad
just pH to 8.5. Autoclave for 15 min at 15 psi pres
sure–121°C. Cool to 25°C. Aseptically add 1.0mL of
sterile trace salts solution. Mix thoroughly. Asepti
cally distribute into sterile tubes or flasks.
Use: For the cultivation of enterotoxigenic Escheri
chia coli.
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