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Casamino Acids Yeast Extract Lincomycin Medium
[所屬分類:培養基配方] [發布時間:2021-11-11] [發布人:網站管理員2] [閱讀次數:] [返回]
Casamino Acids Yeast
Extract Lincomycin Medium
山(shan)東(dong)拓普生物工程有限公(gong)司 培(pei)養基配方(fang) //ljxinglong.com
Composition per liter:
Casamino acids .................................................20.0g
K2HPO4 ............................................................8.71g
Yeast extract........................................................6.0g
NaCl....................................................................2.5g
Lincomycin solution ....................................... 5.0mL
Trace salts solution ......................................... 1.0mL
pH 8.5 ± 0.2 at 25°C
Lincomycin Solution:
Composition per 5.0mL:
Lincomycin....................................................45.0mg
Preparation of Lincomycin Solution: Add lin
comycin to distilled/deionized water and bring vol
ume to 5.0mL. Mix thoroughly. Filter sterilize.
Trace Salts Solution:
Composition per liter:
MgSO4·7H2O....................................................50.0g
MnCl2· 4H2O ......................................................5.0g
FeCl2 ...................................................................5.0g
Preparation of Trace Salts Solution: Add com
ponents to distilled/deionized water and bring vol
ume to 1.0L. Add sufficient 0.1N H2SO4 to dissolve
components. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept trace salts solution and lincomycin solution, to
distilled/deionized water and bring volume to
994.0mL. Mix thoroughly. Adjust pH to 8.5. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to
25°C. Aseptically add 1.0mL of sterile trace salts so
lution and 5.0mL of sterile lincomycin solution. Mix
thoroughly. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation of heat-labile, toxin-produc
ing enterotoxigenic Escherichia coli.
